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【专题讨论】实验试剂专区

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常驻站友

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各位老师,有谁知道那里可以购到能够穿膜的IP3?我查阅了一些资料,但基本上都是别人赠送的.现在我们迫切需要这类试剂,而且我给国外相关文献的作者发了E-mail，但是目前还没有得到答复．麻烦大家帮忙，能否提供这方面的信息？无论如何，我先谢谢大家了！另外还想请教大家，给国外的作者发送Ｅ－mail时有什么技巧性吗，需要注意什么？

2004-11-06 17:42

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njcpu

入门站友

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The columns were washed with 20 column volumes of binding buffer, and bound proteins were eluted with binding buffer containing 50 µM IP3 (Dojindo)and 0.05% Brij 35.
Specific [3H]IP3 binding of K508A and GST (2 µg) was carried out as described elsewhere (7, 8). For analysis of G224 and R441Q (0.02 µg), specific binding was determined by subtracting nonspecific binding measured in the presence of 1 µM unlabeled IP3 (Dojindo) from total binding obtained with 0.15-9.6 nM [3H]IP3 (PerkinElmer Life Sciences). Specific IP3 binding of G224 and R441Q (0.1 µg) was assessed as described elsewhere (7, 8). The competitive effect of G224 and R441Q (0.781-50 µg/ml) was assayed by directly adding them to binding mixtures containing 0.96 or 9.6 nM [3H]IP3 and cerebellar microsomes (40 µg) prepared from ddY mice (8-10 weeks old; Nippon SLC), as described elsewhere

2004-11-07 19:29